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Everything about Sanger sequencing
Method of DNA sequencing developed in 1977
Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. After first being developed by Frederick Sanger and colleagues in 1977, it became the most widely used sequencing method for approximately 40 years. An automated instrument using slab gel electrophoresis and fluorescent labels was first commercialized by Applied Biosystems in March 1987. Later, automated slab gels were replaced with automated capillary array electrophoresis. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Sanger sequencingA 5-minute orientation
- Skim a recent paper: Mastering DNA chromatogram analysis in Sanger sequencing for reliable clinical analysisInfluential work since 2022
- Pick up a classic book: The handbook of plant mutation screeningThe most-reprinted book on the subject
- Read the landmark paper: Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR ampliconsCited 3,067 times
- Go sideways: Illumina dye sequencingA closely related topic
- Go sideways: SequencingA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons
EditR: A Method to Quantify Base Editing from Sanger Sequencing
Targeted Next-Generation Sequencing can Replace Sanger Sequencing in Clinical Diagnostics
Guidelines for Sanger sequencing and molecular assay monitoring
A Sequel to Sanger: amplicon sequencing that scales
CRISP-ID: decoding CRISPR mediated indels by Sanger sequencing
Microfabricated bioprocessor for integrated nanoliter-scale Sanger DNA sequencing
Systematic Evaluation of Sanger Validation of Next-Generation Sequencing Variants
🆕 Influential since 2022
Mastering DNA chromatogram analysis in Sanger sequencing for reliable clinical analysis
Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods
Genomic surveillance of SARS-CoV-2 Spike gene by sanger sequencing
Mass Screening of SARS-CoV-2 Variants using Sanger Sequencing Strategy in Hiroshima, Japan
Systematic Comparison of Computational Tools for Sanger Sequencing-Based Genome Editing Analysis
📚 Books worth reading
The handbook of plant mutation screeningKhalid Meksem · 2009
Principles of gene manipulation and genomicsS. B. Primrose · 2006
Elastic filaments of the cellGerald H. Pollack · 2000- 📕RhinovirusesDavid A. Jans, Reena Ghildyal · 2015
Clinical bioinformaticsR. J. Trent · 2007
The Peopling of NewfoundlandJohn J. Mannion · 1977- 📕C. elegans Protein Database WormPepSanger Centre (Hinxton, Cambridge, England), Genome Sequencing Center · 1993
Selected papers of Frederick SangerFrederick Sanger · 1996
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
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- Dideoxynucleotide Dideoxynucleotides are chain-elongating inhibitors of DNA polymerase, used in the Sanger method for DNA sequencing. They are also known as 2',3' because…
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- Genomics Genomics is an interdisciplinary field of molecular biology focusing on the structure, function, evolution, mapping, and editing of genomes. A genome is…