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Everything about Pyrosequencing
Method of DNA sequencing
Pyrosequencing is a non-electrophoretic DNA sequencing method based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released, hence, the name given it. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: PyrosequencingA 5-minute orientation
- Skim a recent paper: In-herd prevalence of Fasciola hepatica and Calicophoron / Paramphistomum spp. infections in German dairy cows with comparison of two coproscopical methods and establishment of real-time pyrosequencing for rumen fluke species differentiationInfluential work since 2022
- Pick up a classic book: Molecular analysis and genome discoveryThe most-reprinted book on the subject
- Read the landmark paper: Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental ScaleCited 3,653 times
- Go sideways: SequencingA closely related topic
- Go sideways: Pål NyrénA closely related topic
📖 Definitions
Noun A technique used to sequence DNA using chemiluminescent enzymatic reactions.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Pyrosequencing-Based Assessment of Soil pH as a Predictor of Soil Bacterial Community Structure at the Continental Scale
Pyrosequencing enumerates and contrasts soil microbial diversity
Error-correcting barcoded primers for pyrosequencing hundreds of samples in multiplex
Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates
A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses
454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants
Accuracy and quality of massively parallel DNA pyrosequencing
Fast UniFrac: facilitating high-throughput phylogenetic analyses of microbial communities including analysis of pyrosequencing and PhyloChip data
🆕 Influential since 2022
In-herd prevalence of Fasciola hepatica and Calicophoron / Paramphistomum spp. infections in German dairy cows with comparison of two coproscopical methods and establishment of real-time pyrosequencing for rumen fluke species differentiation
Definition of the Prognostic Role of MGMT Promoter Methylation Value by Pyrosequencing in Newly Diagnosed IDH Wild-Type Glioblastoma Patients Treated with Radiochemotherapy: A Large Multicenter Study
Applying fluorescence spectroscopy and DNA pyrosequencing with 2D-COS and co-occurrence network to deconstruct dynamical DOM degradation of air-land-water sources in an urban river
DNA methylation‐based age prediction with bloodstains using pyrosequencing and random forest regression
Pyrosequencing: Current forensic methodology and future applications—a review
📚 Books worth reading
Molecular analysis and genome discoveryRalph Rapley · 2004
Molecular diagnosticsGeorge P. Patrinos, Wilhelm Ansorge · 2009
Bacterial pangenomicsAlessio Mengoni, Marco Galardini · 2015
PharmacogenomicsFederico Innocenti, Ron H. N. Van Schaik · 2005
Single nucleotide polymorphismsAnton A. Komar · 2009
Principles of gene manipulation and genomicsS. B. Primrose · 2006
Pyrosequencing protocolsSharon Marsh · 2007- 📕PyrosequencingUlrich Lehmann, Jorg Tost · 2015
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Sequencing In genetics and biochemistry, sequencing means to determine the primary structure (sometimes incorrectly called the primary sequence) of an unbranched…
- Pål Nyrén Pål Nyrén (born 1955[not verified in body]) was, as of June 2009, a biochemistry professor at the Royal Institute of Technology (KTH), Stockholm.[needs…
- 454 Life Sciences 454 Life Sciences was a biotechnology company based in Branford, Connecticut, that specialized in high-throughput DNA sequencing. It was acquired by Roche…
- Mostafa Ronaghi Mostafa Ronaghi (Persian: مصطفی رونقی; born 1968[not verified in body]) is an Iranian molecular biologist, specializing in DNA sequencing methodology.…
- Massively parallel sequencing Massively parallel sequencing (MPS) is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing;…
- Sulfate adenylyltransferase Sulfate adenylyltransferase (EC 2.7.7.4) is an enzyme that catalyzes the first of two chemical reactions that convert adenosine triphosphate to 3'-pho…
- Pyrophosphate In chemistry, pyrophosphates are phosphorus oxyanions that contain two phosphorus atoms in a P−O−P linkage. A number of pyrophosphate salts exist, such…
- Illumina dye sequencing Illumina dye sequencing is a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. The reversible terminated chemistry…