Everything about Protein microarray
Device for tracking proteins
A protein microarray is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel. The chip consists of a support surface such as a glass slide, nitrocellulose membrane, bead, or microtitre plate, to which an array of capture proteins is bound. Probe molecules, typically labeled with a fluorescent dye, are added to the array. Any reaction between the probe and the immobilised protein emits a fluorescent signal that is read by a laser scanner. Protein microarrays are rapid, automated, economical, and highly sensitive, consuming small quantities of samples and reagents. The concept and methodology of protein microarrays was first introduced and illustrated in antibody microarrays in 1983 in a scientific publication and a series of patents. The high-throughput technology behind the protein microarray was relatively easy to develop since it is based on the technology developed for DNA microarrays, which have become the most widely used microarrays. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Protein microarrayA 5-minute orientation
- Skim a recent paper: Insights Into Glycobiology and the Protein-Glycan Interactome Using Glycan Microarray TechnologiesInfluential work since 2022
- Pick up a classic book: Microarray Image and Data AnalysisThe most-reprinted book on the subject
- Read the landmark paper: High-throughput prediction of protein antigenicity using protein microarray dataCited 488 times
- Go sideways: Antibody microarrayA closely related topic
- Go sideways: MicroarrayA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🏆 Landmark papers (most cited)
High-throughput prediction of protein antigenicity using protein microarray data
Protein microarray technology
Recent Developments in Protein Microarray Technology
Progress in protein and antibody microarray technology
Protein microarray technology
Next generation of protein microarray support materials:
Protein microarray technology
Advances in functional protein microarray technology
🆕 Influential since 2022
Insights Into Glycobiology and the Protein-Glycan Interactome Using Glycan Microarray Technologies
Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis
Miniaturized microarray-format digital ELISA enabled by lithographic protein patterning
Fast and Sensitive Detection of Protein Markers Using an All-Printing Photonic Crystal Microarray via Fingertip Blood
Novel serological biomarker panel using protein microarray can distinguish active TB from latent TB infection
📚 Books worth reading
- 📕Microarray Image and Data AnalysisLuis Rueda · 2014
Exploration And Analysis Of Dna Microarray And Other Highdimensional DataDhammika Amaratunga · 2013
Peptide microarraysMarina Cretich, Marcella Chiari · 2009
Microarray Methods and ProtocolsRobert S. Matson · 2008
Applying Genomic and Proteomic Microarray Technology in Drug DiscoveryRobert S. Matson · 2004- 📕Functional protein microarrays in drug discoveryPaul F. Predki · 2007
Tissue ProteomicsBrian Liu · 2008- 📕Bioinformatics and drug discoveryRichard S. Larson · 2005
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Antibody microarray An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies…
- Microarray Microarray at Wikipedia's sister projects Definitions from Wiktionary Textbooks from Wikibooks Resources from Wikiversity A microarray is a multiplex lab-on-a-c…
- Cell-free protein array Cell-free protein array technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This…
- Biochip In molecular biology, biochips are engineered substrates ("miniaturized laboratories") that can host large numbers of simultaneous biochemical reactions…
- Reverse phase protein lysate microarray A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression…
- Peptide microarray A peptide microarray (also commonly known as peptide chip or peptide epitope microarray) is a collection of peptides displayed on a solid surface, usually…
- Immunoprecipitation Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular…
- Methods to investigate protein–protein interactions There are many methods to investigate protein–protein interactions which are the physical contacts of high specificity established between two or more…