Everything about Microarray
Small-scale two-dimensional array of samples on a solid support
A microarray is a multiplex lab-on-a-chip. Its purpose is to simultaneously detect the expression of thousands of biological interactions. It is a two-dimensional array on a solid substrate—usually a glass slide or silicon thin-film cell—that assays (tests) large amounts of biological material using high-throughput screening miniaturized, multiplexed and parallel processing and detection methods. The concept and methodology of microarrays was first introduced and illustrated in antibody microarrays by Tse Wen Chang in 1983 in a scientific publication and a series of patents. The "gene chip" industry started to grow significantly after the 1995 Science Magazine article by the Ron Davis and Pat Brown labs at Stanford University. With the establishment of companies, such as Affymetrix, Agilent, Applied Microarrays, Arrayjet, Illumina, and others, the technology of DNA microarrays has become the most sophisticated and the most widely used, while the use of protein, peptide and carbohydrate microarrays is expanding. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: MicroarrayA 5-minute orientation
- Skim a recent paper: Feature selection methods on gene expression microarray data for cancer classification: A systematic reviewInfluential work since 2022
- Pick up a classic book: Gene expression studies using affymetrix microarraysThe most-reprinted book on the subject
- Read the landmark paper: limma powers differential expression analyses for RNA-sequencing and microarray studiesCited 39,460 times
- Go sideways: Protein microarrayA closely related topic
- Go sideways: Reverse phase protein lysate microarrayA closely related topic
📖 Definitions
Noun Any of several devices, containing a two-dimensional array of small quantities of biological material, used for various types of assay; the assay itself
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
limma powers differential expression analyses for RNA-sequencing and microarray studies
GSVA: gene set variation analysis for microarray and RNA-Seq data
Linear Models and Empirical Bayes Methods for Assessing Differential Expression in Microarray Experiments
Adjusting batch effects in microarray expression data using empirical Bayes methods
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs
TM4: A Free, Open-Source System for Microarray Data Management and Analysis
Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray
🆕 Influential since 2022
Feature selection methods on gene expression microarray data for cancer classification: A systematic review
Validation of the new EPIC DNA methylation microarray (900K EPIC v2) for high-throughput profiling of the human DNA methylome
Trophoblast Cell Surface Antigen 2 Expression in Human Tumors: A Tissue Microarray Study on 18,563 Tumors
Gene selection for microarray data classification via multi-objective graph theoretic-based method
Molecular Diagnostic Yield of Exome Sequencing and Chromosomal Microarray in Cerebral Palsy
📚 Books worth reading
Gene expression studies using affymetrix microarraysHinrich Gohlmann, Willem Talloen · 2009
Exploration And Analysis Of Dna Microarray And Other Highdimensional DataDhammika Amaratunga · 2013
Microarray Methods and ProtocolsRobert S. Matson · 2008
A biologist's guide to analysis of DNA microarray dataSteen Knudsen · 2002
DNA microarrays and gene expressionPierre Baldi · 2002
Applying Genomic and Proteomic Microarray Technology in Drug DiscoveryRobert S. Matson · 2004- 📕Cytogenetic AbnormalitiesSusan Mahler Zneimer · 2014
Molecular DiagnosticsHarald Seitz · 2013
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Protein microarray A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function…
- Reverse phase protein lysate microarray A reverse phase protein lysate microarray (RPMA) is a protein microarray designed as a dot-blot platform that allows measurement of protein expression…
- Antibody microarray An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies…
- DNA microarray A DNA microarray (also commonly known as a DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA…
- Reverse transfection Reverse transfection is a technique for the transfer of genetic material into cells. As DNA is printed on a glass slide for the transfection process (the…
- Biochip In molecular biology, biochips are engineered substrates ("miniaturized laboratories") that can host large numbers of simultaneous biochemical reactions…
- Cell-free protein array Cell-free protein array technology produces protein microarrays by performing in vitro synthesis of the target proteins from their DNA templates. This…
- Immunoprecipitation Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular…