Everything about DNA microarray
Collection of microscopic DNA spots attached to a solid surface
A DNA microarray is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or to genotype multiple regions of a genome. Each DNA spot contains picomoles of a specific DNA sequence, known as probes. These can be a short section of a gene or other DNA element that are used to hybridize a cDNA or cRNA sample under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target. The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was published in 1981. It was invented by Patrick O. Brown. An example of its application is in SNPs arrays for polymorphisms in cardiovascular diseases, cancer, pathogens and GWAS analysis. It is also used for the identification of structural variations and the measurement of gene expression. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: DNA microarrayA 5-minute orientation
- Skim a recent paper: Validation of the new EPIC DNA methylation microarray (900K EPIC v2) for high-throughput profiling of the human DNA methylomeInfluential work since 2022
- Pick up a classic book: Molecular analysis and genome discoveryThe most-reprinted book on the subject
- Read the landmark paper: Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA MicroarrayCited 7,157 times
- Go sideways: Microarray analysis techniquesA closely related topic
- Go sideways: RNA spike-inA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
Wireless Electrochemical DNA Microarray Sensor
DNA microarray technologies for measuring protein–DNA interactions
Microfluidic DNA microarray analysis: A review
DNA Microarray Analysis of Complex Biologic Processes
Evaluation of toxicity of the mycotoxin citrinin using yeast ORF DNA microarray and Oligo DNA microarray
DNA analysis with multiplex microarray-enhanced PCR
Experimental Design of DNA Microarray Experiments
🆕 Influential since 2022
Validation of the new EPIC DNA methylation microarray (900K EPIC v2) for high-throughput profiling of the human DNA methylome
Validation of a DNA methylation microarray for 285,000 CpG sites in the mouse genome
A new filter-based gene selection approach in the DNA microarray domain
Dipodal Silanes Greatly Stabilize Glass Surface Functionalization for DNA Microarray Synthesis and High-Throughput Biological Assays
Recent Application of DNA Microarray Techniques to Diagnose Infectious Disease
📚 Books worth reading
Molecular analysis and genome discoveryRalph Rapley · 2004
Integrated microfabricated biodevicesAndras Guttman · 2001
A biologist's guide to analysis of DNA microarray dataSteen Knudsen · 2002
DNA microarrays and gene expressionPierre Baldi · 2002
Microarray Methods and ProtocolsRobert S. Matson · 2008
Applying Genomic and Proteomic Microarray Technology in Drug DiscoveryRobert S. Matson · 2004
Genomics in drug discovery and developmentDimitri Semizarov · 2008
Guide to analysis of DNA microarray dataSteen Knudsen · 2004
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Microarray analysis techniques Microarray analysis techniques are used in interpreting the data generated from experiments on DNA (Gene chip analysis), RNA, and protein microarrays,…
- RNA spike-in An RNA spike-in is an RNA transcript of known sequence and quantity used to calibrate measurements in RNA hybridization assays, such as DNA microarray…
- Suspension array technology Suspension array technology (or SAT) is a high throughput, large-scale, and multiplexed screening platform used in molecular biology. SAT has been widely…
- MAGIChip MAGIChips, also known as "microarrays of gel-immobilized compounds on a chip" or "three-dimensional DNA microarrays", are devices for molecular hybridization…
- Illumina Methylation Assay The Illumina Methylation Assay using the Infinium I platform uses 'BeadChip' technology[clarification needed] to generate a comprehensive genome-wide profiling…
- Tiling array Tiling arrays are a subtype of microarray chips. Like traditional microarrays, they function by hybridizing labeled DNA or RNA target molecules to probes…
- Transcriptomics technologies Transcriptomics technologies are the techniques used to study an organism's transcriptome, the sum of all of its RNA transcripts. The information content…
- Massively parallel signature sequencing Massive parallel signature sequencing (MPSS) is a procedure that is used to identify and quantify mRNA transcripts, resulting in data similar to serial…