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Everything about Operon
Group of open reading frames under the same regulation
In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter. The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo splicing to create monocistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product. The result of this is that the genes contained in the operon are either expressed together or not at all. Several genes must be co-transcribed to define an operon. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: OperonA 5-minute orientation
- Skim a recent paper: Metagenomic and Metatranscriptomic Insights into Population Diversity of Microcystis Blooms: Spatial and Temporal Dynamics of mcy Genotypes, Including a Partial Operon That Can Be Abundant and ExpressedInfluential work since 2022
- Pick up a classic book: The organization and control of the rplJL-rpoBC operon in Escherichia coliThe most-reprinted book on the subject
- Read the landmark paper: A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory systemCited 1,396 times
- Go sideways: RepressorA closely related topic
- Go sideways: Lac operonA closely related topic
📖 Definitions
Noun A unit of genetic material that functions in a coordinated manner by means of an operator, a promoter, and structural genes that are transcribed together.
🏆 Landmark papers (most cited)
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli
Improved single and multicopy lac-based cloning vectors for protein and operon fusions
rRNA Operon Copy Number Reflects Ecological Strategies of Bacteria
Inactivation of the dlt Operon inStaphylococcus aureus Confers Sensitivity to Defensins, Protegrins, and Other Antimicrobial Peptides
rrndb: the Ribosomal RNA Operon Copy Number Database
Crystal Structure of the Lactose Operon Repressor and Its Complexes with DNA and Inducer
Biochemical Method for Inserting New Genetic Information into DNA of Simian Virus 40: Circular SV40 DNA Molecules Containing Lambda Phage Genes and the Galactose Operon of Escherichia coli
🆕 Influential since 2022
Metagenomic and Metatranscriptomic Insights into Population Diversity of Microcystis Blooms: Spatial and Temporal Dynamics of mcy Genotypes, Including a Partial Operon That Can Be Abundant and Expressed
Short‐ and long‐read metabarcoding of the eukaryotic rRNA operon: Evaluation of primers and comparison to shotgun metagenomics sequencing
PHB production from food waste hydrolysates by Halomonas bluephagenesis Harboring PHB operon linked with an essential gene
Species-Wide Phylogenomics of the Staphylococcus aureus Agr Operon Revealed Convergent Evolution of Frameshift Mutations
Operon
📚 Books worth reading
- 📕The organization and control of the rplJL-rpoBC operon in Escherichia coliGerard F. Barry · 1980
- 📕Simple Mathematical Models of Gene Regulatory DynamicsMichael C. Mackey, Moisés Santillán · 2016
GUS protocolsSean R. Gallagher · 1992
The OperonJeffrey H. Miller, William S. Reznikoff · 1980- 📕The Operon · 1978
The lac OperonBenno Müller-Hill · 1996- 📕The kilB operon of promiscuous plasmid RK2Valeri Jean Thomson · 1994
The OperonJeffrey H. Miller, William S. Reznikoff · 1978
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Repressor In molecular genetics, a repressor is a DNA- or RNA-binding protein that inhibits the expression of one or more genes by binding to the operator or associated…
- Lac operon The lactose operon (lac operon) is an operon required for the transport and metabolism of lactose in E. coli and many other enteric bacteria. Although…
- Inducer In molecular biology, an inducer is a molecule that regulates gene expression. An inducer functions in two ways; namely: By disabling repressors. The gene…
- Gal operon The gal operon is a prokaryotic operon, which encodes enzymes necessary for galactose metabolism. Repression of gene expression for this operon works via…
- Silencer (genetics) In genetics, a silencer is a DNA sequence capable of binding transcription regulation factors, called repressors. DNA contains genes and provides the template…
- Trp operon The trp operon is a group of genes that are transcribed together, encoding the enzymes that produce the amino acid tryptophan in bacteria. The trp operon…
- L-arabinose operon The L-arabinose operon, also called the ara or araBAD operon, is an operon required for the breakdown of the five-carbon sugar L-arabinose in Escherichia…
- Gene structure Gene structure is the organisation of specialised sequence elements within a gene. Genes contain most of the information necessary for living cells to…