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Everything about Variants of PCR
Various uses and techniques of polymerase chain reaction in the laboratory
Variants of PCR represent a diverse array of techniques that have evolved from the basic polymerase chain reaction (PCR) method, each tailored to specific applications in molecular biology, such as genetic analysis, DNA sequencing, and disease diagnosis, by modifying factors like primer design, temperature conditions, and enzyme usage. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Variants of PCRA 5-minute orientation
- Skim a recent paper: Multiplex PCR Assays for Identifying all Major Severe Acute Respiratory Syndrome Coronavirus 2 VariantsInfluential work since 2022
- Pick up a classic book: Principles of gene manipulation and genomicsThe most-reprinted book on the subject
- Read the landmark paper: Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data SetsCited 6,398 times
- Go sideways: Polymerase chain reaction optimizationA closely related topic
- Go sideways: Polymerase chain reactionA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🏆 Landmark papers (most cited)
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
Quantification of splice variants using real-time PCR
Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants
Nucleoprotein gene analysis of fixed and street rabies virus variants using RT-PCR
Quantitative Analysis of Copy Number Variants Based on Real‐Time LightCycler PCR
Real‐Time quantitative PCR analysis of factor XI mRNA variants in human platelets
Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants
Direct identification by PCR of EBV types and variants in clinical samples
🆕 Influential since 2022
Multiplex PCR Assays for Identifying all Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants
Tracking SARS-CoV-2 variants through pandemic waves using RT-PCR testing in low-resource settings
New RT-PCR Assay for the Detection of Current and Future SARS-CoV-2 Variants
Comparative Analysis of Five Multiplex RT-PCR Assays in the Screening of SARS-CoV-2 Variants
Evaluation of RT-PCR assays for detection of SARS-CoV-2 variants of concern
📚 Books worth reading
Principles of gene manipulation and genomicsS. B. Primrose · 2006- 📕Animal biodiversity and prediction & prevention of emerging diseasesSociety for Tropical Veterinary Medicine. Meeting · 2008
In silico tools for gene discoveryBing Yu, Marcus Hinchcliffe · 2011
Forensic DNA typing protocolsAngel Carracedo · 2005- 📕RNA mappingM. Lucrecia Alvarez, Mahtab Nourbakhsh · 2014
Cancer cell cultureIan A. Cree · 2011
Ovarian cancerAnastasia Malek, Oleg Tchernitsa · 2013- 📕Molecular diagnostics for melanomaMagdalena Thurin, Francesco M. Marincola · 2014
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
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- Reverse transcription polymerase chain reaction Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called…