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Everything about Primer dimer
Byproduct in PCR formed by primer annealing to itself
A primer dimer (PD) is a potential by-product in the polymerase chain reaction (PCR), a common biotechnological method. As its name implies, a PD consists of two primer molecules that have attached (hybridized) to each other because of strings of complementary bases in the primers. As a result, the DNA polymerase amplifies the PD, leading to competition for PCR reagents, thus potentially inhibiting amplification of the DNA sequence targeted for PCR amplification. In quantitative PCR, PDs may interfere with accurate quantification. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Primer dimerA 5-minute orientation
- Skim a recent paper: Both aerosol and primer dimer breakdown for straightforward genotyping based on an integrated immunochromatographic biosensorInfluential work since 2022
- Pick up a classic book: Principles of gene manipulation and genomicsThe most-reprinted book on the subject
- Read the landmark paper: AutoDimer: a screening tool for primer-dimer and hairpin structuresCited 435 times
- Go sideways: Polymerase chain reaction optimizationA closely related topic
- Go sideways: Primer (molecular biology)A closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
AutoDimer: a screening tool for primer-dimer and hairpin structures
The elimination of primer-dimer accumulation in PCR
Elimination of Primer–Dimer Artifacts and Genomic Coamplification Using a Two-Step SYBR Green I Real-Time RT-PCR
Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE)
Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation.
CRISPR/Cas9 bridged recombinase polymerase amplification with lateral flow biosensor removing potential primer-dimer interference for robust Staphylococcus aureus assay
Studies on primer-dimer formation in polymerase chain reaction (PCR)
An Internal Amplification Control System Based on Primer-Dimer Formation for PCR Product Detection by DNA Hybridization
🆕 Influential since 2022
📚 Books worth reading
Principles of gene manipulation and genomicsS. B. Primrose · 2006
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
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