Everything about Transgene
Gene or genetic material that has been transferred from one organism to another
A transgene is a gene that has been transferred naturally, or by any of a number of genetic engineering techniques, from one organism to another. The introduction of a transgene, in a process known as transgenesis, has the potential to change the phenotype of an organism. Transgene describes a segment of DNA containing a gene sequence that has been isolated from one organism and is introduced into a different organism. This non-native segment of DNA may either retain the ability to produce RNA or protein in the transgenic organism or alter the normal function of the transgenic organism's genetic code. In general, the DNA is incorporated into the organism's germ line. For example, in higher vertebrates this can be accomplished by injecting the foreign DNA into the nucleus of a fertilized ovum. This technique is routinely used to introduce human disease genes or other genes of interest into strains of laboratory mice to study the function or pathology involved with that particular gene. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: TransgeneA 5-minute orientation
- Skim a recent paper: The sound of silence: Transgene silencing in mammalian cell engineeringInfluential work since 2022
- Pick up a classic book: Transgenic plantsThe most-reprinted book on the subject
- Read the landmark paper: Human Induced Pluripotent Stem Cells Free of Vector and Transgene SequencesCited 2,054 times
- Go sideways: Exogenous DNAA closely related topic
- Go sideways: Gene knock-inA closely related topic
📖 Definitions
Noun A gene from one species introduced into the genome of another by transgenesis.
🏆 Landmark papers (most cited)
Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome
Control of Memory Formation Through Regulated Expression of a CaMKII Transgene
Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.
Localized Igf-1 transgene expression sustains hypertrophy and regeneration in senescent skeletal muscle
Retracted: Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana
An RNA-Dependent RNA Polymerase Gene in Arabidopsis Is Required for Posttranscriptional Gene Silencing Mediated by a Transgene but Not by a Virus
Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila
🆕 Influential since 2022
The sound of silence: Transgene silencing in mammalian cell engineering
Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Durability of transgene expression after rAAV gene therapy
Intestinal transgene delivery with native E. coli chassis allows persistent physiological changes
Interindividual variability in transgene mRNA and protein production following adeno-associated virus gene therapy for hemophilia A
📚 Books worth reading
Transgenic plantsShain-dow Kung · 1992- 📕Applied Mathematics and Omics to Assess Crop Genetic Resources for Climate Change Adaptive TraitsAbdallah Bari, Ardeshir B. Damania · 2016
Methods for risk assessment of transgenic plantsKlaus Ammann · 1999- 📕Un-Making Environmental ActivismDoerthe Rosenow · 2017
Genetically Modified OrganismsYves Tourte · 2019
Genetically engineered marine organismsRaymond A. Zilinskas, Peter J. Balint · 1998- 📕Transgenic Plants and CropsGeorge C. Khachatourians, Y. H. Hui · 2002
Frankenstein's catEmily Anthes · 2013
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Exogenous DNA Exogenous DNA is DNA originating outside the organism of concern or study. Exogenous DNA can be found naturally in the form of partially degraded fragments…
- Gene knock-in In molecular cloning and biology, a gene knock-in (abbreviation: KI) refers to a genetic engineering method that involves the one-for-one substitution…
- Genetically modified mouse A genetically modified mouse, genetically engineered mouse model (GEMM) or transgenic mouse is a mouse (Mus musculus) that has had its genome altered through…
- Transgenesis Transgenesis is the process of introducing an exogenous gene—called a transgene—into a living organism so that the organism will exhibit a new property…
- Genetically modified animal Genetically modified animals are animals that have been genetically modified for a variety of purposes including producing drugs, enhancing yields, increasing…
- Gene targeting Gene targeting is a biotechnological tool used to change the DNA sequence of an organism (hence it is a form of genome editing). It is based on the natural…
- Mouse models of breast cancer metastasis Breast cancer metastatic mouse models are experimental approaches in which mice are genetically manipulated to develop a mammary tumor leading to distant…
- Genetic engineering techniques Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, delete, and modify DNA at multiple…