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Everything about Transfer DNA binary system
A transfer DNA (T-DNA) binary system is a pair of plasmids consisting of a T-DNA binary vector and a vir helper plasmid. The two plasmids are used together to produce genetically modified plants. They are artificial vectors that have been derived from the naturally occurring Ti plasmid found in bacterial species of the genus Agrobacterium, such as A. tumefaciens. The binary vector is a shuttle vector, so-called because it is able to replicate in multiple hosts. Wikipedia →
🧠Start here: a 6-step reading path
- Read the overview: Transfer DNA binary systemA 5-minute orientation
- Pick up a classic book: Principles of gene manipulation and genomicsThe most-reprinted book on the subject
- Read the landmark paper: A binary-BAC system for plant transformation with high-molecular-weight DNACited 151 times
- Go sideways: Plant transformation vectorA closely related topic
- Go sideways: Transfer DNAA closely related topic
đź“– Definitions
No dictionary entry for this exact phrase.
🏆 Landmark papers (most cited)
đź“š Books worth reading
Principles of gene manipulation and genomicsS. B. Primrose · 2006
Plant fungal pathogensMelvin D. Bolton, Bart P. H. J. Thomma · 2012
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
đź”— Related topics
- Plant transformation vector Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used…
- Transfer DNA The transfer DNA (abbreviated T-DNA) is the transferred DNA of the tumor-inducing (Ti)/root-inducing (Ri) plasmid of plant-pathogenic bacteria such as…
- Ti plasmid A tumour inducing (Ti) plasmid is a plasmid found in pathogenic species of Agrobacterium sensu lato, including A. tumefaciens, Rhizobium rhizogenes, A…
- Agrobacterium tumefaciens Agrobacterium tumefaciens is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. It is a rod-shaped, Gram-negativ…
- Gene delivery Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Gene delivery must reach the genome of the host…
- Agrobacterium Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. Agrobacterium…
- Bacterial conjugation Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between…
- EHA101 EHA101 was one of the first and most widely used Agrobacterium helper plasmid for plant gene transfer. Created in 1985 in the laboratory of Mary-Dell Chilton…