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Everything about Spliceosome
Molecular machine that removes intron RNA from the primary transcript
A spliceosome is a large complex of proteins and RNA (ribonucleoprotein) that functions to remove introns from pre-mRNA, a process called RNA splicing. Humans and many other eukaryotes have two splicesomes, the major spliceosome, which removes 99%+ introns, and the minor spliceosome which is involved in fewer. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: SpliceosomeA 5-minute orientation
- Skim a recent paper: Transcriptome-wide splicing network reveals specialized regulatory functions of the core spliceosomeInfluential work since 2022
- Pick up a classic book: The nucleolusThe most-reprinted book on the subject
- Read the landmark paper: The Spliceosome: Design Principles of a Dynamic RNP MachineCited 2,512 times
- Go sideways: Small nuclear RNAA closely related topic
- Go sideways: Minor spliceosomeA closely related topic
📖 Definitions
Noun A dynamic complex of RNA and protein subunits that removes introns from precursor mRNA.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
The Spliceosome: Design Principles of a Dynamic RNP Machine
Spliceosome Structure and Function
A day in the life of the spliceosome
Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things
The spliceosome deposits multiple proteins 20–24 nucleotides upstream of mRNA exon–exon junctions
Large-Scale Proteomic Analysis of the Human Spliceosome
RNA Splicing by the Spliceosome
Comprehensive proteomic analysis of the human spliceosome
🆕 Influential since 2022
Transcriptome-wide splicing network reveals specialized regulatory functions of the core spliceosome
Structural basis of branch site recognition by the human spliceosome
Dominant variants in major spliceosome U4 and U5 small nuclear RNA genes cause neurodevelopmental disorders through splicing disruption
Structural studies of the spliceosome: Bridging the gaps
Mechanism of exon ligation by human spliceosome
📚 Books worth reading
The nucleolusMark O. J. Olson · 2004- 📕Complementable spliceosomes and the role of exon sequences in pre-mRNA splicingAnnette Suzanne Parent · 1989
Mass spectrometryA. L. Burlingame · 2005
mRNA processing and metabolismDaniel R. Schoenberg · 2004
Rna Protein Interaction ProtocolsLin Ren-jang · 2008- 📕Spliceosomal Pre-MRNAKlemens J. Hertel · 2016
Spliceosomal pre-mRNA splicingKlemens J. Hertel · 2014- 📕Evidence the spliceosome is a ribozymeNima Shah · 2004
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Small nuclear RNA Small nuclear RNA (snRNA) is a class of small RNA molecules that are found within the splicing speckles and Cajal bodies of the cell nucleus in eukaryotic…
- Minor spliceosome The minor spliceosome is a complex of RNA and proteins (ribonucleoprotein complex) that catalyses the removal (splicing) of an atypical class of introns…
- SnRNP snRNPs (pronounced "snurps"), or small nuclear ribonucleoproteins, are RNA-protein complexes that combine with unmodified pre-mRNA and various other proteins…
- Snurposome Snurposomes are a type of granular structure that are found in the nuclei of oocytes. They are found in animals including mammals, amphibians, and insects…
- U4 spliceosomal RNA The U4 small nuclear Ribo-Nucleic Acid (U4 snRNA) is a non-coding RNA component of the major or U2-dependent spliceosome – a eukaryotic molecular machine…
- Prp8 Prp8 refers to both the Prp8 protein and Prp8 gene. Prp8's name originates from its involvement in pre-mRNA processing. The Prp8 protein is a large, highly…
- U11 spliceosomal RNA The U11 snRNA (small nuclear ribonucleic acid) is an important non-coding RNA in the minor spliceosome protein complex, which activates the alternative…
- U6 spliceosomal RNA U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclear ribonucleoprotein), an RNA-protein complex that combines with…