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Everything about Polymerase chain reaction
Laboratory technique to multiply a DNA sample for study
The polymerase chain reaction (PCR) is a laboratory method widely used to amplify copies of specific DNA sequences rapidly, to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Polymerase chain reactionA 5-minute orientation
- Skim a recent paper: MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments GuidelinesInfluential work since 2022
- Pick up a classic book: Making PCRThe most-reprinted book on the subject
- Read the landmark paper: Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodCited 168,064 times
- Go sideways: History of polymerase chain reactionA closely related topic
- Go sideways: Taq polymeraseA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
A new mathematical model for relative quantification in real-time RT-PCR
Analyzing real-time PCR data by the comparative CT method
Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
🆕 Influential since 2022
MIQE 2.0: Revision of the Minimum Information for Publication of Quantitative Real-Time PCR Experiments Guidelines
Pathologic complete response (pCR) to neoadjuvant treatment with or without atezolizumab in triple-negative, early high-risk and locally advanced breast cancer: NeoTRIP Michelangelo randomized study
Minimizing errors in RT-PCR detection and quantification of SARS-CoV-2 RNA for wastewater surveillance
Droplet-based digital PCR (ddPCR) and its applications
Evaluation of Serum Mucorales Polymerase Chain Reaction (PCR) for the Diagnosis of Mucormycoses: The MODIMUCOR Prospective Trial
📚 Books worth reading
Making PCRPaul Rabinow · 1996
Virus ManiaTorsten Engelbrecht, Claus Köhnlein, MD · 2021
A laboratory guide for in vivo studies of DNA methylation and protein/DNA interactionsH. P. Saluz · 1990
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
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