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Everything about Optical sectioning

Imaging of focal planes within a thick sample

Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. Wikipedia →

🧭 Start here: a 6-step reading path

  1. Read the overview: Optical sectioningA 5-minute orientation
  2. Skim a recent paper: Optical sectioning methods in three-dimensional bioimagingInfluential work since 2022
  3. Pick up a classic book: Final report, grant NAG-1-882, UW 144-AH14The most-reprinted book on the subject
  4. Read the landmark paper: Optical sectioning microscopyCited 712 times
  5. Go sideways: Total internal reflection fluorescence microscopeA closely related topic
  6. Go sideways: Light sheet fluorescence microscopyA closely related topic

📖 Definitions

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🗂️ The subject at a glance

  1. Optical sectioning in traditional light microscopes
  2. Techniques for improving optical sectioning
  3. Alternatives

🏆 Landmark papers (most cited)

🆕 Influential since 2022

📚 Books worth reading

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🔗 Related topics