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Everything about Fluorescence microscope
Optical microscope that uses fluorescence and phosphorescence
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. A fluorescence microscope is any microscope that uses fluorescence to generate an image, whether it is a simple setup like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Fluorescence microscopeA 5-minute orientation
- Skim a recent paper: Ångström-resolution fluorescence microscopyInfluential work since 2022
- Pick up a classic book: Introduction to FluorescenceThe most-reprinted book on the subject
- Read the landmark paper: Two-Photon Laser Scanning Fluorescence MicroscopyCited 8,533 times
- Go sideways: Super-resolution microscopyA closely related topic
- Go sideways: Vertico spatially modulated illuminationA closely related topic
📖 Definitions
Noun A microscope that uses fluorescence to form an image
🏆 Landmark papers (most cited)
Two-Photon Laser Scanning Fluorescence Microscopy
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
Use of nuclepore filters for counting bacteria by fluorescence microscopy
Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution
🆕 Influential since 2022
Ångström-resolution fluorescence microscopy
Multiphoton fluorescence microscopy for in vivo imaging
A cryogenic, coincident fluorescence, electron, and ion beam microscope
A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach
Deep learning–enabled filter-free fluorescence microscope
📚 Books worth reading
Introduction to FluorescenceDavid M. Jameson · 2014
Multi-modality microscopyHanry Yu · 2006
Light microscopic techniques in biology and medicineJ. James · 1976
Cells and tissues by light and electron microscopyEdmund Bertil Sandborn · 1970
FluorescenzmikroskopieMax Haitinger · 1938
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Super-resolution microscopy Super-resolution microscopy is a series of super-resolution imaging techniques in optical microscopy that allow such images to have resolutions higher…
- Vertico spatially modulated illumination Vertico spatially modulated illumination (Vertico-SMI) is the fastest[citation needed] light microscope for the 3D analysis of complete cells in the nanometer…
- Immunofluorescence Immunofluorescence (IF) is a light microscopy-based technique that allows detection and localization of a wide variety of target biomolecules within a…
- Microscopy Microscopy is the technical field of using microscopes to view subjects too small to be seen with the naked eye (objects that are not within the resolution…
- Optical microscope The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate…
- Total internal reflection fluorescence microscope A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers…
- Confocal microscopy Confocal microscopy is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole…
- Fluorescence imaging Fluorescence imaging is a type of non-invasive imaging technique that can help visualize biological processes taking place in a living organism. Fluorescence…