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Everything about ELISA
Method to detect an antigen using an antibody and enzyme
The enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical biochemical assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand in a liquid sample using antibodies directed against the ligand to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: ELISAA 5-minute orientation
- Skim a recent paper: Emerging ELISA derived technologies for in vitro diagnosticsInfluential work since 2022
- Pick up a classic book: Prague CounterpointThe most-reprinted book on the subject
- Read the landmark paper: Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GCited 3,114 times
- Go sideways: ImmunoassayA closely related topic
- Go sideways: ELISpotA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🏆 Landmark papers (most cited)
Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G
Enzyme-Linked Immunosorbent Assay, Elisa
Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA)
Plasmonic ELISA for the ultrasensitive detection of disease biomarkers with the naked eye
Science with the space-based interferometer eLISA. II: gravitational waves from cosmological phase transitions
Comparison of three analytical platforms for quantification of the neurofilament light chain in blood samples: ELISA, electrochemiluminescence immunoassay and Simoa
A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA
Paper‐Based ELISA
🆕 Influential since 2022
Emerging ELISA derived technologies for in vitro diagnostics
An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken
Development of a streptavidin-bridged enhanced sandwich ELISA based on self-paired nanobodies for monitoring multiplex Salmonella serogroups
Development of a biotinylated nanobody for sensitive detection of aflatoxin B1 in cereal via ELISA
📚 Books worth reading
Prague CounterpointBrock Thoene · 1989
Munich SignatureBrock Thoene · 1990
Vienna PreludeBrock Thoene · 1989
The Bitter KingdomRae Carson · 2013- 📕Per ElisaLucio Lugnani · 1990
The ELISA guidebookJohn R. Crowther · 2000
Munich signatureBrock Thoene · 1990
The ELISA guidebookJ. R. Crowther · 2009
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Immunoassay An immunoassay (IA) is a biochemical test that measures the presence or concentration of a macromolecule or a small molecule in a solution through the…
- ELISpot The enzyme-linked immunosorbent spot (ELISpot) is a type of assay that focuses on quantitatively measuring the frequency of cytokine secretion for a single…
- Mass spectrometric immunoassay Mass spectrometric immunoassay (MSIA) is a rapid method is used to detect and/ or quantify antigens and or antibody analytes. This method uses an analyte…
- Lateral flow test A lateral flow test (LFT), is an assay also known as a lateral flow immunochromatographic test (ICT). It is a simple device intended to detect the presence…
- Flow-through test Flow-through tests or immunoconcentration assays are a type of diagnostic assay that allows users to test for the presence of a biomarker, usually a specific…
- Antibody microarray An antibody microarray (also known as antibody array) is a specific form of protein microarray. In this technology, a collection of captured antibodies…
- Assay An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively…
- Magnetic immunoassay Magnetic immunoassay (MIA) is a type of diagnostic immunoassay using magnetic beads as labels in lieu of conventional enzymes (ELISA), radioisotopes (RIA)…