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Everything about Assay

Measure the amount of a target entity

An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit. Wikipedia →

🧭 Start here: a 6-step reading path

  1. Read the overview: AssayA 5-minute orientation
  2. Skim a recent paper: DPPH Radical Scavenging AssayInfluential work since 2022
  3. Pick up a classic book: Toxicity testing using microorganismsThe most-reprinted book on the subject
  4. Read the landmark paper: Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assaysCited 47,055 times
  5. Go sideways: ELISAA closely related topic
  6. Go sideways: ImmunoassayA closely related topic

📖 Definitions

Noun Trial, attempt.

Noun Examination and determination; test.

Noun The qualitative or quantitative chemical analysis of something.

Noun Trial by danger or by affliction; adventure; risk; hardship; state of being tried.

🗂️ The subject at a glance

  1. Etymology
  2. General steps
  3. Assay types based on the nature of the assay process
  4. Assay types based on the targets being measured
  5. Quality
  6. List of BioAssay databases

🏆 Landmark papers (most cited)

🆕 Influential since 2022

📚 Books worth reading

Ranked by number of editions on Open Library, a rough measure of how lasting a book is.

🔗 Related topics