Everything about ClpX

🧭 Start here: a 6-step reading path

1. Read the overview: ClpXA 5-minute orientation
2. Skim a recent paper: Tag-Dependent Substrate Selection of ClpX Underlies Secondary Differentiation of Chlamydia trachomatisInfluential work since 2022
3. Pick up a classic book: Characterization of the N-terminal domain of the molecular chaperone ClpxThe most-reprinted book on the subject
4. Read the landmark paper: Proteomic Discovery of Cellular Substrates of the ClpXP Protease Reveals Five Classes of ClpX-Recognition SignalsCited 546 times
5. Go sideways: Endopeptidase ClpA closely related topic
6. Go sideways: HslVUA closely related topic

🏆 Landmark papers (most cited)

• Proteomic Discovery of Cellular Substrates of the ClpXP Protease Reveals Five Classes of ClpX-Recognition Signals

  Julia M. Flynn, Saskia B. Neher, Yong-In Kim · 2003 · Molecular Cell · 546 citations

• Alternative roles of ClpX and ClpP in Staphylococcus aureus stress tolerance and virulence

  Dorte Frees, Saara N. A. Qazi, Philip J. Hill · 2003 · Molecular Microbiology · 312 citations

• ClpX(P) Generates Mechanical Force to Unfold and Translocate Its Protein Substrates

  Rodrigo A. Maillard, Gheorghe Chistol, Maya Sen · 2011 · Cell · 288 citations

• ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli. Sequence and in vivo activities.

  S Gottesman, W.P. Clark, V de Crecy-Lagard · 1993 · Journal of Biological Chemistry · 263 citations

• Pore loops of the AAA+ ClpX machine grip substrates to drive translocation and unfolding

  Andreas Martin, Tania A Baker, Robert T Sauer · 2008 · Nature Structural & Molecular Biology · 251 citations

• Disassembly of the Mu transposase tetramer by the ClpX chaperone.

  I Levchenko, L Luo, T A Baker · 1995 · Genes & Development · 245 citations

• Structures of Asymmetric ClpX Hexamers Reveal Nucleotide-Dependent Motions in a AAA+ Protein-Unfolding Machine

  Steven E. Glynn, Andreas Martin, Andrew R. Nager · 2009 · Cell · 237 citations

• Isolation and characterization of ClpX, a new ATP-dependent specificity component of the Clp protease of Escherichia coli.

  D Wojtkowiak, C Georgopoulos, M Zylicz · 1993 · Journal of Biological Chemistry · 212 citations

🆕 Influential since 2022

• Tag-Dependent Substrate Selection of ClpX Underlies Secondary Differentiation of Chlamydia trachomatis

  Nicholas A. Wood, Abigail R. Swoboda, Amanda M. Blocker · 2022 · mBio · 23 citations

• ClpP/ClpX deficiency impairs mitochondrial functions and mTORC1 signaling during spermatogenesis

  Chenxi Guo, Yuan Xiao, Jingkai Gu · 2023 · Communications Biology · 17 citations

• The ClpX protease is essential for inactivating the CI master repressor and completing prophage induction in Staphylococcus aureus

  Mohammed A. Thabet, José R. Penadés, Andreas F. Haag · 2023 · Nature Communications · 15 citations

• Disruption of the ATP-dependent unfoldase ClpX reverses antifungal resistance in Cryptococcus neoformans

  M. Woods, A. Bermas, B. Ball · 2025 · Nature Communications · 11 citations

• ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans

  Vivek Gurung, Indranil Biswas · 2022 · Journal of Bacteriology · 11 citations

📚 Books worth reading

• 📕Characterization of the N-terminal domain of the molecular chaperone ClpxUrszula A. Wojtyra · 2003
• 📕Towards understanding the functions of the GroEL and ClpX chaperone systems in Escherichia coliToni Davidson · 2004

Ranked by number of editions on Open Library, a rough measure of how lasting a book is.

🔗 Related topics

• Endopeptidase Clp Endopeptidase Clp (EC 3.4.21.92, endopeptidase Ti, caseinolytic protease, protease Ti, ATP-dependent Clp protease, ClpP, Clp protease). This enzyme catalyses…
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• ATP-dependent Clp protease proteolytic subunit ATP-dependent Clp protease proteolytic subunit (ClpP) is an enzyme that in humans is encoded by the CLPP gene. This protein is an essential component to…
• AAA proteins AAA (ATPases Associated with diverse cellular Activities) proteins (more commonly referred to as "triple-A ATPases") are a large group of protein family…
• PSMC6 26S protease regulatory subunit S10B, also known as 26S proteasome AAA-ATPase subunit Rpt4, is an enzyme that in humans is encoded by the PSMC6 gene. This…
• PSMC2 26S protease regulatory subunit 7, also known as 26S proteasome AAA-ATPase subunit Rpt1, is an enzyme that in humans is encoded by the PSMC2 gene This…
• PSMC3 26S protease regulatory subunit 6A, also known as 26S proteasome AAA-ATPase subunit Rpt5, is an enzyme that in humans is encoded by the PSMC3 gene. This…
• Proteasome Proteasomes are essential protein complexes responsible for the degradation of proteins by proteolysis, a chemical reaction that breaks peptide bonds.…