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Everything about Bio-layer interferometry
Optical biosensing technology
Bio-layer interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling. Alongside surface plasmon resonance (SPR), BLI is one of few widely available label-free biosensing technologies, a detection style that yields more information in less time than traditional processes. The technology relies on the phase shift-wavelength correlation created between interference patterns off two unique surfaces on the tip of a biosensor. BLI has significant applications in quantifying binding strength, measuring protein interactions, and identifying properties of reaction kinetics, such as rate constants and reaction rates. Wikipedia →
🧠Start here: a 6-step reading path
- Read the overview: Bio-layer interferometryA 5-minute orientation
- Skim a recent paper: Bio-based phytic acid and urea interfacial layer by layer assembly for flame-retardant cottonInfluential work since 2022
- Read the landmark paper: Designing binding kinetic assay on the bio-layer interferometry (BLI) biosensor to characterize antibody-antigen interactionsCited 134 times
- Go sideways: Methods to investigate protein–protein interactionsA closely related topic
- Go sideways: Grating-coupled interferometryA closely related topic
đź“– Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Designing binding kinetic assay on the bio-layer interferometry (BLI) biosensor to characterize antibody-antigen interactions
Strategies Using Bio-Layer Interferometry Biosensor Technology for Vaccine Research and Development
Identification of natural compounds as SARS-CoV-2 entry inhibitors by molecular docking-based virtual screening with bio-layer interferometry
Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions
Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes
Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
High throughput detection of antibody self-interaction by bio-layer interferometry
High-Throughput Kinetic Screening of Hybridomas to Identify High-Affinity Antibodies Using Bio-Layer Interferometry
🆕 Influential since 2022
Bio-based phytic acid and urea interfacial layer by layer assembly for flame-retardant cotton
Bio-Layer Interferometry-Based SELEX and Label-Free Detection of Patulin Using Generated Aptamer
Discovery of drug targets based on traditional Chinese medicine microspheres (TCM-MPs) fishing strategy combined with bio-layer interferometry (BLI) technology
An antibody-free bio-layer interferometry biosensor for immunoglobulin G1 detection in human serum by using molecularly imprinted polynorepinephrine
An Experimental Framework for Developing Point-of-Need Biosensors: Connecting Bio-Layer Interferometry and Electrochemical Impedance Spectroscopy
đź“š Books worth reading
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đź”— Related topics
- Methods to investigate protein–protein interactions There are many methods to investigate protein–protein interactions which are the physical contacts of high specificity established between two or more…
- Grating-coupled interferometry Grating-coupled interferometry (GCI) is a biophysical characterization method mainly used in biochemistry and drug discovery for label-free analysis of…
- Single colour reflectometry Single colour reflectometry (SCORE), formerly known as imaging Reflectometric Interferometry (iRIf) and 1-lambda Reflectometry, is a physical method based…
- Multi-parametric surface plasmon resonance Multi-parametric surface plasmon resonance (MP-SPR) is based on surface plasmon resonance (SPR), an established real-time label-free method for biomolecular…
- IRIS (biosensor) Interferometric reflectance imaging sensor (IRIS), formerly known as the spectral reflectance imaging biosensor (SRIB), is a system that can be used as…
- Biosensor A biosensor (or nanobiosensor in the nanoscale) is an analytical device, used for the detection of a chemical substance, that combines a biological component…
- Surface plasmon resonance microscopy Surface plasmon resonance microscopy (SPRM), also called surface plasmon resonance imaging (SPRI), is a label free analytical tool that combines the surface…
- Surface plasmon resonance Surface plasmon resonance (SPR) is a phenomenon that occurs where electrons in a thin metal sheet become excited by light that is directed to the sheet…