Everything about Single-cell sequencing
Examines sequence information from individual cells
Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. For example, in cancer, sequencing the DNA of individual cells can give information about mutations carried by small populations of cells. In development, sequencing the RNAs expressed by individual cells can give insight into the existence and behavior of different cell types. In microbial systems, a population of the same species can appear genetically clonal. Still, single-cell sequencing of RNA or epigenetic modifications can reveal cell-to-cell variability that may help populations rapidly adapt to survive in changing environments. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Single-cell sequencingA 5-minute orientation
- Skim a recent paper: Single‐cell RNA sequencing technologies and applications: A brief overviewInfluential work since 2022
- Pick up a classic book: Campbell biologyThe most-reprinted book on the subject
- Read the landmark paper: SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingCited 25,673 times
- Go sideways: G&T-seqA closely related topic
- Go sideways: List of single cell omics methodsA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🏆 Landmark papers (most cited)
SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
The promise of single-cell sequencing
Microfluidic single-cell whole-transcriptome sequencing
Single-Cell Sequencing of Brain Cell Transcriptomes and Epigenomes
Introduction to Single‐Cell RNA Sequencing
Single-cell sequencing in stem cell biology
The trajectory of microbial single-cell sequencing
Single Cell RNA Sequencing in Atherosclerosis Research
🆕 Influential since 2022
📚 Books worth reading
Campbell biologyLisa A. Urry, Michael L. Cain · 2015
Biological AgingTrygve O. Tollefsbol · 2005
Theory and Practice of ComputationShin-ya Nishizaki · 2013
DNA nanotechnologyGiampaolo Zuccheri · 2011
BiocodeDawn Field · 2015
DNA replicationSonya Vengrova, Jacob Z. Dalgaard · 2009- 📕LymphomaRalf Küppers · 2012
Molecular diagnosticsGeorge P. Patrinos, Wilhelm Ansorge · 2009
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- G&T-seq G&T-seq (short for single cell genome and transcriptome sequencing) is a novel form of single-cell sequencing technique allowing one to simultaneously…
- List of single cell omics methods A list of more than 100 different single cell sequencing (omics) methods have been published. The large majority of methods are paired with short-read…
- Spatial transcriptomics Spatial transcriptomics, or spatially resolved transcriptomics, is a method that captures positional context of transcriptional activity within intact…
- RNA-Seq RNA-Seq (short for RNA sequencing) is a next-generation sequencing (NGS) technique used to quantify and identify RNA molecules in a biological sample,…
- Single cell epigenomics Single cell epigenomics is the study of epigenomics (the complete set of epigenetic modifications on the genetic material of a cell) in individual cells…
- Epigenomics Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous…
- Multiple displacement amplification Multiple displacement amplification (MDA) is a DNA amplification technique. This method can rapidly amplify minute amounts of DNA samples to a reasonable…
- Bisulfite sequencing Bisulfite sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern…