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Everything about Reverse transcriptase
Enzyme which generates DNA
A reverse transcriptase (RT) is an enzyme that uses an RNA molecule as a template to synthesize a complementary DNA molecule, through a process termed reverse transcription. Reverse transcriptases are used by viruses such as HIV and hepatitis B to replicate their genomes, by retrotransposon mobile genetic elements to proliferate within the host genome, and by eukaryotic cells to extend the telomeres at the ends of their linear chromosomes. The process does not violate the flows of genetic information as described by the classical central dogma, but rather expands it to include transfers of information from RNA to DNA. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: Reverse transcriptaseA 5-minute orientation
- Skim a recent paper: A split prime editor with untethered reverse transcriptase and circular RNA templateInfluential work since 2022
- Pick up a classic book: Human Immunodeficiency Virus Reverse TranscriptaseThe most-reprinted book on the subject
- Read the landmark paper: Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data SetsCited 6,397 times
- Go sideways: RetrovirusA closely related topic
- Go sideways: Retroviral ribonuclease HA closely related topic
📖 Definitions
Noun A polymerase that catalyzes the formation of DNA from an RNA template.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
Crystal Structure at 3.5 Å Resolution of HIV-1 Reverse Transcriptase Complexed with an Inhibitor
Development of a Real-Time Reverse Transcriptase PCR Assay for Type A Influenza Virus and the Avian H5 and H7 Hemagglutinin Subtypes
Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction
Broadly Reactive and Highly Sensitive Assay for Norwalk-Like Viruses Based on Real-Time Quantitative Reverse Transcription-PCR
🆕 Influential since 2022
A split prime editor with untethered reverse transcriptase and circular RNA template
Molecular Docking, Validation, Dynamics Simulations, and Pharmacokinetic Prediction of Phytochemicals Isolated From Croton dichogamus Against the HIV-1 Reverse Transcriptase
Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line
Approved HIV reverse transcriptase inhibitors in the past decade
The regulations of telomerase reverse transcriptase (TERT) in cancer
📚 Books worth reading
Human Immunodeficiency Virus Reverse TranscriptaseStuart LeGrice, Matthias Gotte · 2013
The PCR techniquePaul D. Siebert · 1998- 📕Gene cloning and analysis by RT-PCRPaul D. Siebert, James W. Larrick · 1998
Retroviral Reverse TranscriptasesSIMON LITVAK · 1996- 📕Assays for reverse transcriptase activity and p53 proteinJohan Lennerstrand · 1996
Differential-display reverse transcription-PCR (DDRT-PCR)Sergio Colonna-Romano · 1998
PCR/RT-PCR in situ light and electron microscopy · 2002
Reverse transcriptase inhibitors in HIV/AIDS therapy · 2006
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- Retrovirus A retrovirus is a virus that inserts a DNA copy of its RNA genome into the DNA of a host cell that it invades, thus changing the genome of that cell. After…
- Retroviral ribonuclease H Retroviral ribonuclease H (retroviral RNase H) is a catalytic domain of the retroviral reverse transcriptase (RT) enzyme. The RT enzyme is used to generate…
- Hepatitis B virus DNA polymerase Hepatitis B virus DNA polymerase is a hepatitis B viral protein. It is a DNA polymerase that can use either DNA or RNA templates and a ribonuclease H that…
- Ribonuclease H Ribonuclease H (abbreviated RNase H or RNH) is a family of non-sequence-specific endonuclease enzymes that catalyze the cleavage of RNA in an RNA/DNA substrate…
- Complementary DNA In genetics, complementary DNA (cDNA) is DNA that was reverse transcribed (via reverse transcriptase) from an RNA (e.g., messenger RNA or microRNA). cDNA…
- NASBA (molecular biology) Nucleic acid sequence-based amplification, commonly referred to as NASBA, is a method in molecular biology which is used to produce multiple copies of…
- Hepadnaviridae Hepadnaviridae is a family of viruses. Humans, apes, and birds serve as natural hosts. The family contains five genera. Its best-known member is hepatitis…
- Riboviria Riboviria is a realm of viruses that includes all viruses that use a homologous RNA-dependent polymerase for replication. It includes RNA viruses that…