Everything about RefSeq

🧭 Start here: a 6-step reading path

1. Read the overview: RefSeqA 5-minute orientation
2. Skim a recent paper: NCBI RefSeq: reference sequence standards through 25 years of curation and annotationInfluential work since 2022
3. Pick up a classic book: The NCBI handbookThe most-reprinted book on the subject
4. Read the landmark paper: Reference sequence (RefSeq) database at NCBI: current status, taxonomic expansion, and functional annotationCited 6,368 times
5. Go sideways: Donna R. MaglottA closely related topic
6. Go sideways: Consensus CDS ProjectA closely related topic

🏆 Landmark papers (most cited)

• Reference sequence (RefSeq) database at NCBI: current status, taxonomic expansion, and functional annotation

  Nuala A. O'Leary, Mathew W. Wright, J. Rodney Brister · 2015 · Nucleic Acids Research · 6,368 citations

• NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins

  K. D. Pruitt, T. Tatusova, D. R. Maglott · 2007 · Nucleic Acids Research · 2,700 citations

• NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins

  K. D. Pruitt · 2004 · Nucleic Acids Research · 1,298 citations

• RefSeq: expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation

  Wenjun Li, Kathleen R O’Neill, Daniel H Haft · 2020 · Nucleic Acids Research · 1,177 citations

• NCBI Reference Sequences (RefSeq): current status, new features and genome annotation policy

  K. D. Pruitt, T. Tatusova, G. R. Brown · 2011 · Nucleic Acids Research · 1,039 citations

• RefSeq: an update on prokaryotic genome annotation and curation

  Daniel H Haft, Michael DiCuccio, Azat Badretdin · 2017 · Nucleic Acids Research · 972 citations

• RefSeq: an update on mammalian reference sequences

  Kim D. Pruitt, Garth R. Brown, Susan M. Hiatt · 2013 · Nucleic Acids Research · 903 citations

• RefSeq and LocusLink: NCBI gene-centered resources

  K. D. Pruitt · 2001 · Nucleic Acids Research · 616 citations

🆕 Influential since 2022

• NCBI RefSeq: reference sequence standards through 25 years of curation and annotation

  Tamara Goldfarb, Vamsi K Kodali, Shashikant Pujar · 2024 · Nucleic Acids Research · 465 citations

• RefSeq and the prokaryotic genome annotation pipeline in the age of metagenomes

  Daniel H Haft, Azat Badretdin, George Coulouris · 2023 · Nucleic Acids Research · 111 citations

• Lifting of the 1,000 wheat exome project SNPs from Triticum aestivum cv. Chinese Spring assembly RefSeq v1.0 to RefSeq v2.1

  Akshaya Vasudevan, Sylvie Cloutier · 2023 · BMC Research Notes · 7 citations

• Revisiting and corrections to the annotated SRSF3 (SRp20) gene structure and RefSeq sequences from the human and mouse genomes

  Lulu Yu, Vladimir Majerciak, Rong Jia · 2023 · Cell Insight · 6 citations

• Differences in GenBank and RefSeq annotations may affect genomics data interpretation for Pseudomonas putida KT2440

  Guilherme Marcelino Viana de Siqueira, Thomas Eng, Aindrila Mukhopadhyay · 2025 · mSphere · 4 citations

📚 Books worth reading

• 📕The NCBI handbookNational Center for Biotechnology Information (U.S.) · 2002

Ranked by number of editions on Open Library, a rough measure of how lasting a book is.

🔗 Related topics

• Donna R. Maglott Donna R. Maglott is a staff scientist at the National Center for Biotechnology Information known for her research on large-scale genomics projects, including…
• Consensus CDS Project The Consensus Coding Sequence (CCDS) Project is a collaborative effort to maintain a dataset of protein-coding regions that are identically annotated on…
• European Bioinformatics Institute The European Bioinformatics Institute (EMBL-EBI) is an intergovernmental organization (IGO) which, as part of the European Molecular Biology Laboratory…
• List of biological databases Biological databases are stores of biological information. The journal Nucleic Acids Research regularly publishes special issues on biological databases…
• Sequence Read Archive The Sequence Read Archive (SRA, previously known as the Short Read Archive) is a bioinformatics database that provides a public repository for DNA sequencing…
• European Nucleotide Archive The European Nucleotide Archive (ENA) is a repository providing free and unrestricted access to annotated DNA and RNA sequences. It also stores complementary…
• Centrosomal protein 15 Centrosomal protein 15 is a protein of uncertain function which in humans is encoded by the gene CEP15 (previously C3orf14). The human gene is located…
• Expression Atlas The Expression Atlas is a database maintained by the European Bioinformatics Institute that provides information on gene expression patterns from RNA-Seq…