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Everything about ChIP sequencing
Method used to analyze protein interactions with DNA
ChIP-sequencing, also known as ChIP-seq, is a method used to analyze protein interactions with DNA. ChIP-seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify the binding sites of DNA-associated proteins. It can be used to map global binding sites precisely for any protein of interest. Previously, ChIP-on-chip was the most common technique utilized to study these protein–DNA relations. Wikipedia →
🧭 Start here: a 6-step reading path
- Read the overview: ChIP sequencingA 5-minute orientation
- Skim a recent paper: GWAS of Reproductive Traits in Large White Pigs on Chip and Imputed Whole-Genome Sequencing DataInfluential work since 2022
- Pick up a classic book: DNA microarraysThe most-reprinted book on the subject
- Read the landmark paper: ChIP-seq: Using high-throughput sequencing to discover protein–DNA interactionsCited 505 times
- Go sideways: CUT&RUN sequencingA closely related topic
- Go sideways: Chromatin immunoprecipitationA closely related topic
📖 Definitions
No dictionary entry for this exact phrase.
🗂️ The subject at a glance
🏆 Landmark papers (most cited)
ChIP-seq: Using high-throughput sequencing to discover protein–DNA interactions
Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP)
Progress in Ion Torrent semiconductor chip based sequencing
An interactive environment for agile analysis and visualization of ChIP-sequencing data
GimmeMotifs: a de novo motif prediction pipeline for ChIP-sequencing experiments
Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing
Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice
🆕 Influential since 2022
GWAS of Reproductive Traits in Large White Pigs on Chip and Imputed Whole-Genome Sequencing Data
Angiogenesis-on-a-chip coupled with single-cell RNA sequencing reveals spatially differential activations of autophagy along angiogenic sprouts
The design, validation, and utility of the “ZJU CottonSNP40K” liquid chip through genotyping by target sequencing
Development and Validation of a 54K Genome-Wide Liquid SNP Chip Panel by Target Sequencing for Dairy Goat
Development of breeder chip for gene detection and molecular-assisted selection by target sequencing in wheat
📚 Books worth reading
DNA microarrays · 2005
Ranked by number of editions on Open Library, a rough measure of how lasting a book is.
🔗 Related topics
- CUT&RUN sequencing CUT&RUN sequencing, also known as cleavage under targets and release using nuclease, is a method used to analyze protein interactions with DNA. CUT&RUN…
- Chromatin immunoprecipitation Chromatin immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA…
- ChIL-sequencing ChIL sequencing (ChIL-seq), also known as Chromatin Integration Labeling sequencing, is a method used to analyze protein interactions with DNA. ChIL-sequencing…
- CUT&Tag sequencing CUT&Tag-sequencing, also known as cleavage under targets and tagmentation, is a method used to analyze protein interactions with DNA. CUT&Tag-sequencing…
- ChIP-exo ChIP-exo is a chromatin immunoprecipitation based method for mapping the locations at which a protein of interest (transcription factor) binds to the genome…
- ChIP-on-chip ChIP-on-chip (also known as ChIP-chip) is a technology that combines chromatin immunoprecipitation ('ChIP') with DNA microarray ("chip"). Like regular…
- ChIA-PET Chromatin Interaction Analysis by Paired-End Tag Sequencing (ChIA-PET or ChIA-PETS) is a technique in molecular biology that incorporates chromatin immunoprecip…
- Peak calling Peak calling is a computational method used to identify areas in a genome that have been enriched with aligned reads as a consequence of performing a ChIP-seque…